Mechanisms of tissue factor induction by Porphyromonas gingivalis in human endothelial cells
김소희, 정지연, 김원재, 김옥준, 김영, 강인철,
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김소희 ( Kim So-Hee ) - Chonnam National University Dental Science Research Institute
정지연 ( Jung Ji-Yeon ) - Chonnam National University Dental Science Research Institute
김원재 ( Kim Won-Jae ) - Chonnam National University Dental Science Research Institute
김옥준 ( Kim Ok-Joon ) - Chonnam National University Dental Science Research Institute
김영 ( Kim Young ) - Chonnam National University Dental Science Research Institute
강인철 ( Kang In-Chol ) - Chonnam National University Dental Science Research Institute
Abstract
Associations between periodontal infection and cardiovascular disease have been documented. Porphyromonas gingivalis is a well-established periodontal pathogen, and tissue factor (TF) is a key initiator of the coagulation cascade. In this context, P. gingivalis has been reported to enhance TF expression in human endothelial cells. The present study investigated the underlying mechanisms of TF induction by P. gingivalis in human umbilical vein endothelial cells. P. gingivalis increased TF expression in a dose- and time-dependent manner. Not only live bacteria but also glutaraldehyde-fixed bacteria increased TF expression to the same extent. However, sonicates of P. gingivalis did not induce TF expression. Cytochalasin D and SMIFH2, which are inhibitors of actin polymerization and actin nucleation, respectively, inhibited the TF expression induced by P. gingivalis . Finally, TF production was decreased or increased in the presence of various signaling inhibitors, including mitogen-activated protein kinases. These results suggest that P. gingivalis induces endothelial TF expression by a bacterial internalization-dependent mechanism and through diverse signal transduction mechanisms.
키워드
Porphyromonas gingivalis; Tissue factor; Endothelial cells
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